![]() Store the activated stain at room temperature. Dissolve the crystals by mixing end-over-end for one minute, then allow it to remain at room temperature for five minutes before use. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. (3pts) Use the Kaleidoscope ladder key included in the gel image above to determine the size (in kD) of each band (peptide) in the ladder. Based on the gel image above, complete the table below. For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. Precision Plus Protein Standards Kaleidoscope BIO RAD 1. Protein Migration Chart MW, kD 2 5 0 15 0 10 0 75 5 0 37 2 5 20 15 10 WesternC Dual Color Kaleidoscope Unstained All Blue Precision Plus Protein Standards Migration patterns of Precision Plus Protein standards on Criterion Tris-HCl pH 8.8 gels. A couple of bands in my protein standard are missing on the gel. Department of Transportation (DOT) shipping requirements. the dye front reaches the bottom of the gel. Precision Plus Protein Dual Color Standards, 500 l. Bioz Stars score: 96/100, based on 1 PubMed citations. Prestained markers are brighter for visual robustness throughout western blotting to accurately estimate MW, monitor electrophoresis and transfer efficiency. Stable-store non-activated or activated stain at room temperature for up to one yearīecause the supplied activator crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Bio-Rad molecular weight marker precision plus protein kaleidoscope prestained protein ladder Molecular Weight Marker Precision Plus Protein Kaleidoscope Prestained Protein Ladder, supplied by Bio-Rad, used in various techniques.Easy to use-add and dissolve activator crystals before first use, then simply add reagent to stain gels.Multiple applications-qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry.Compatible-suitable for staining 1D and 2D polyacrylamide gels and nitrocellulose and PVDF membranes (multiple protocols provided in the instructions).Minimal additional reagents-water washes only no acid-fixative or methanol destain solutions required.Safe-non-corrosive to skin, non-flammable, and safe to ship and store until activated.Sensitive-detect down to 9 ng of protein per band in 15 minutes.GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |